Over the past decade, the CRISPR-Cas9 system has transformed genetic engineering by offering a precise and efficient way to modify genomes. While knockout strategies—where gene function is disrupted by introducing frameshift mutations—are widely used, the ability to knock in defined DNA sequences has unlocked even greater potential. CRISPR knock-in technology enables the insertion of point mutations, fluorescent tags, selectable markers, or entire genes into endogenous loci, providing powerful tools for disease modeling, therapeutic development, and functional genomics.
Read more: https://www.ubigene.us/applica....tion/crispr-knock-in

Delivering genetic material into mammalian cells is a central step in modern molecular biology, yet the choice of method often determines whether an experiment succeeds or fails. While adherent cells are generally receptive to chemical transfection reagents or electroporation, suspension cell types—such as lymphocytes, T-cell leukemia lines, and hematopoietic progenitors—are notoriously resistant. Their lack of adhesion, tendency to aggregate, and negatively charged membrane surface reduce the effectiveness of conventional methods.
In these cases, lentiviral transduction has become the preferred strategy. Lentiviruses, members of the retrovirus family, possess the ability to infect both dividing and non-dividing cells and to integrate their genetic cargo stably into the host genome. This combination of high efficiency, stable expression, and broad tropism makes them especially well suited for suspension cells.
Read more: https://www.ubigene.us/service..../virus/lentivirus-pa

Gene knockout (KO) technology has become one of the most widely used approaches in modern molecular biology. By completely disrupting the function of a target gene, researchers can explore its role in cellular pathways, disease progression, and drug responses. Although the advent of CRISPR-Cas systems has significantly simplified the process, generating a robust KO cell line still requires careful planning and optimization. From sgRNA design to validation, every step influences the final outcome.
This article provides a comprehensive overview of strategies to optimize gene knockout experiments, highlights common pitfalls, and offers practical recommendations for ensuring reliable results.
Read more: https://www.ubigene.us/applica....tion/knockout-cell-o

CRISPR screening has become a cornerstone of functional genomics, allowing researchers to investigate gene function at scale and uncover mechanisms behind complex biological processes. While experimental design is critical, the real challenge often begins once the sequencing data are generated. From read mapping to hit identification, each analytical step carries nuances that can affect the final interpretation. This article highlights common challenges in CRISPR screening analysis and provides practical guidance on how to address them.
https://www.ubigene.us/applica....tion/crispr-screenin

Unlocking Gene Function: A Practical Guide to Functional Screening with CRISPR Libraries

In the era of genome editing, CRISPR-based functional screening has emerged as a powerful strategy for understanding gene function on a genome-wide scale. By leveraging large-scale CRISPR libraries, researchers can perform unbiased, high-throughput investigations that pinpoint genes associated with diverse cellular phenotypes—from drug resistance to immune evasion.
This article provides a clear, practical overview of how to design, build, and execute a functional screening platform using CRISPR libraries. We’ll walk through the foundational concepts, typical screening setups, selection strategies, and real-world applications.
Read more: https://www.ubigene.us/applica....tion/crispr-screenin