Gene knockout (KO) technology has become one of the most widely used approaches in modern molecular biology. By completely disrupting the function of a target gene, researchers can explore its role in cellular pathways, disease progression, and drug responses. Although the advent of CRISPR-Cas systems has significantly simplified the process, generating a robust KO cell line still requires careful planning and optimization. From sgRNA design to validation, every step influences the final outcome.
This article provides a comprehensive overview of strategies to optimize gene knockout experiments, highlights common pitfalls, and offers practical recommendations for ensuring reliable results.
Read more: https://www.ubigene.us/applica....tion/knockout-cell-o
