CRISPR screening has become a cornerstone of functional genomics, allowing researchers to investigate gene function at scale and uncover mechanisms behind complex biological processes. While experimental design is critical, the real challenge often begins once the sequencing data are generated. From read mapping to hit identification, each analytical step carries nuances that can affect the final interpretation. This article highlights common challenges in CRISPR screening analysis and provides practical guidance on how to address them.
https://www.ubigene.us/applica....tion/crispr-screenin
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