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The biochar-induced priming effects (PEs) were investigated by applying maize straw (C4) derived biochar to eight C3 soils, with a gradient of pH and a sub-gradient of soil organic carbon (SOC). To decipher the physicochemical and microbial mechanisms, we adopted C-isotopic analysis, high-throughput sequencing and multivariate statistical analyses such as random forest (RF) and structure equation modeling (SEM). Negative and neutral PEs were observed up to -48.5% of relative PEs during 28 days of incubation. All the acidic soils exhibit
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