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Detection of HHV-6 by FA-ME led to discontinuation of acyclovir within 12.0 hours in all 12 patients empirically treated with acyclovir. Six of the twelve patients were started on ganciclovir therapy within 6.8 hours; four of which were treated specifically for HHV-6 infection, whereas therapy was discontinued in the remaining 2 patients. CSF parameters were not generally predictive of HHV-6 positivity. The presence of ciHHV-6 was confirmed in 3 of 18 patients able to be tested.Conclusions Five of the 25 patients included in the study we