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Data on the effects of direct particulate matter (PM) exposure on the eyes and the nose are limited. Here, an interleukin (IL)-17/neutrophil-dominant ovalbumin (OVA)/polyinosinic-polycytidylic acid (Poly(IC)) mouse model was used to evaluate the effect of different-sized titanium dioxide (TiO ) particles on the eyes and the nose. We also examined whether IL-17-neutralizing antibody (IL-17Ab) treatment could reverse TiO effects. The nasal cavities and conjunctival sacs of each mouse were challenged with OVA and Poly(IC) to induce neutrop