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gs, and new pharmacological options should be further evaluated. To comprehensively assess associations of site-specific CD4 -T-cell hypomethylation of the CD40-Ligand gene ( ) with disease activity of women with systemic lupus erythematosus (SLE). CpG-sites within the DNA of the promotor and two enhancer regions (n = 22) of were identified and numbered consecutively. The rate of methylated DNA in isolated CD4 -T-cells of women with SLE were quantified for each methylation site by MALDI-TOF. Disease activity was assessed by SLE Disease