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e online version contains supplementary material available at 10.1007/s13205-021-02723-w. The online version contains supplementary material available at 10.1007/s13205-021-02723-w.Hexavalent chromium is a toxic heavy metal getting discharged into the environment and water bodies through various industrial processes. Conventional analysis methods call for expensive equipment and complicated sample pretreatment that made unsuitable for onsite detection. Paper is used as an enzyme immobilization platform because of its property to wick the liquid by capillary
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