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For examining the influence of interfacial property on the SHG effect of low-frequency Lamb wave propagation, the different annealing cycles of the thin adhesive layer (acrylics) are used to simulate minor changes in the interfacial property of the given two-layered plate. It is found that the relative nonlinear acoustic parameter at the MCD decreases monotonically and sensitively with the increment of annealing cycle number, which verifies the quantitative correlation between the SHG efficiency of low-frequency Lamb wave pro