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By comparison of experimental results, we finally verify that the HFMD prediction algorithm proposed in this paper has higher accuracy. At the same time, the early-warning algorithm based on the comparison of threshold has good results.Transfer RNA (tRNA) molecules contain various post-transcriptional modifications that are crucial for tRNA stability, translation efficiency, and fidelity. Besides their canonical roles in translation, tRNAs also originate tRNA-derived small RNAs (tsRNAs), a class of small non-coding RNAs with regulatory f

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