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High-throughput sequencing revealed that genus Thiobacillus was predominant (66.3-90.0%) in all seven reactors. However, at the operational taxonomic unit level, microbial communities at 15 °C and 30 °C were significantly different, indicating that dissimilar strains were cultivated. Our findings suggested that deliberately cultivating cold-adapted denitrifiers helps SAD to achieve high TN removal at psychrophilic temperatures and thus, is important for future applications in practical TN pollution control in urbanized streams.The pr