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Level of IL10, IL6, and IFNγ was determined using an Abcam ELISA kit. Level of IDO was determined using an RnD ELISA kit. The data were analysed using the Mann-whitney tests. Results There are differences in the Tregs population (p = 0.000 0.05). Conclusion High ASA affects of the Tregs population but has no effect on cytokines IL10, IL6, IFNγ, and IDO.Leukemia inhibitory factor (LIF) has played a vital role in a series of reproductive events, including follicle growth, embryo growth and differentiation. However, it is unclear whether th