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Finally, the pathogenicity of both the recombinant virus strains rH120 and rH120/SCS1 was evaluated in SPF chickens. The results showed that the chimeric rH120/SCS1 strain was not pathogenic compared with the wild-type IBV SC021202 strain and the chickens inoculated with rH120/SCS1 could resist challenge infection by IBV SC021202. Taken together, our results indicate that BAC reverse genetic system could be used to rescue IBV in vitro and IBV S1 protein alone might not be the key factor for IBV pathogenicity. KEY POINTS • BAC vector wa

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3) after introduction of PCR technology. There was no significant difference in length of stay postintervention ( = .12). Patients discharged directly from the ED had fewer return visits ( = .02) and received fewer repeat blood cultures ( = .04), and antibiotics were administered on fewer occasions after return ( = .04) postintroduction of PCR technology. With the addition of PCR technology, patients with blood cultures positive for nonpathogenic bacteria received less antibiotics, fewer repeat blood cultures, and fewer repeat E