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The focus of this work described here is to establish a method for monitoring and quantifying the extracellular phosphorylation of Human SHSY5Y undifferentiated neuronal cells by three ectokinases PKA, PKC and Src; these are kinases that are known to be present in the extracellular matrix. Here is demonstrated that a combination of different experimental techniques, including microscopy and electrochemistry, can be used to detect extracellular phosphorylations. Phosphorylation profiles of the three ectokinases, PKA, PKC and

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5, SD = 5.8) with  = -3.220,  = 0.001 and Cohen's  = 0.42. This was due to medium-severely depressed patients At t1, their depressiveness scores decreased significantly (med = 9, M = 9.8; SD = 5.1) compared to t0 (med = 14, M = 15.2; SD = 3.9) with  = -3.730,  ⩽ 0.000 and Cohen's  = 1.2, but others' did not. All other outcomes showed positive descriptive trends. Desire to die-conversations through trained health professionals do not harm palliative patients. Results cautiously suggest temporary improvement. Desire to die-conversations t