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Objective This study investigated a method of preparing corn stover for Irpex lacteus treatment to improve its in vitro rumen degradability under non-sterile conditions. Methods Corn stover was inoculated with Lactobacillus plantarum, Lactobacillus buchneri, and an equal mixture of these strains, and the stover was ensiled for 0, 3, 7, 14 and 28 days. After each period of time, a portion of the silage was sampled, to assess the silage quality, and another portion of the silage was further treated with I. lacteus at 28 °C fo