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Microvesicles (MVs) are large extracellular vesicles differing in size, cargo and composition that share a common mechanism of release from the cells through the direct outward budding of the plasma membrane. They are involved in a variety of physiological and pathological conditions and represent promising biomarkers for diseases. MV heterogeneity together with the lack of specific markers had strongly hampered the development of effective methods for MV isolation and differential centrifugation remains the most used method to purif

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Contemporary studies have discredited the methods used to exclude urinary tract infection (UTI) when treating overactive bladder (OA. Thus we must revisit the OAB phenotype to check that UTI has not been overlooked. To examine the differences in urinary cytokines IL6 and lactoferrin in OAB patients compared to controls, with references to microscopy of urine and enhanced quantitative urine culture. A blinded, prospective cohort study with normal controls using six repeated measures, achieved two-monthly, over 12months. The differences b