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To facilitate in vivo implantation, the CS nanofibers were shaped into three-dimensional macroporous scaffolds and coated with gelatin to improve their mechanical stability. By implanting the scaffolds into rat calvarial defects, it was confirmed the scaffold made of CS nanofibers calcined at 1000 °C was able to enhance new bone formation more efficiently than the scaffolds made of CS nanofibers calcined at 800 °C or 1200 °C. To summarize, calcination temperature could be an effective and useful tool applied to produce CS bioceramic sub

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