Over the past decade, the CRISPR-Cas9 system has transformed genetic engineering by offering a precise and efficient way to modify genomes. While knockout strategies—where gene function is disrupted by introducing frameshift mutations—are widely used, the ability to knock in defined DNA sequences has unlocked even greater potential. CRISPR knock-in technology enables the insertion of point mutations, fluorescent tags, selectable markers, or entire genes into endogenous loci, providing powerful tools for disease modeling, therapeutic development, and functional genomics.
Read more: https://www.ubigene.us/applica....tion/crispr-knock-in
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