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Anaerobic digestion is widely used for sewage sludge stabilization. Different anaerobic digestion methods have different impacts on the speciation and stability of heavy metals in sludge. This study investigated the response of the speciation and stability of heavy metals in sludge to the treatment of mesophilic anaerobic digestion-mechanical dewatering and thermal hydrolysis pre-treatment-thermophilic anaerobic digestion. The results showed that the process of mesophilic anaerobic digestion increased the contents of C