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Intra-day and inter-day precisions were less than 1.2%RSD. Percent recoveries of spiked tyramine in samples were 95 ± 3 to 106 ± 7% (n = 3).Laying hens on the free-range systems are susceptible to challenging situations in relation to the rearing environment. Therefore, this work evaluated how solar radiation influences the behavior of laying hens raised in a free-range system, in the Brazilian Savanna. The activities included data collection of meteorological variables and behavioral analysis of 300 commercial laying hens in rel