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In this fluorometric enzyme assay, the alkyne-triacylated peptide product is rendered fluorescent through a click-chemistry reaction and detected in a multiwell plate format. This technique is relevant to many other acyltransferases that use fatty acid-containing substrates, including phospholipids and acyl-CoA.We explain the step-by-step procedure for culturing and differentiating mouse embryonic stem cells into neuronal lineages, accompanied by a number of assays to characterize t

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