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Mathematical modelling of biological treatment is an effective tool to predict effluent quality. Model calibration is critical to improve the accuracy of simulation, which is normally carried out by fine-tuning the values of parameters according to the practical data. It indicated that huge amount of practical date will be consumed, and it cannot predict the treatment performance of new wastewater. In this study, the main objective was to investigate the feasibility of application BioWin software coupled with determination of sensitive p

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