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Five biomarkers, such as Eubacterium_1 coprostanoligenes, Clostridiales, Phascolarctobacterium, Acidaminococcaceae and Ruminococcaceae_UCG_002 were selected by Lefse analysis. The most positively correlated bacteria were Terrisporobacter and Oscillospiraceae (r = 0.7 and the highly negative correlation was found between Ruminococcaceae_NK4A214_group and [Ruminococcus]_gauvreauii_group (r = 0.64). Thus, hydrogen sulphide destroyed the microbial balance and increased the abundance of harmful bacteria. Our results reveal that hydrogen s

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