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Sensitive, specific and rapid molecular diagnosis of respiratory diseases in animals and humans is critical to facilitate appropriate control measures and treatment. Conventional polymerase chain reaction (PCR)-based molecular diagnostics requires relatively expensive equipment and trained staff, restricting its use to centralized laboratories with significant delays between sample collection and test results. Herein, we report a highly sensitive, rapid, point-of-need, two-stage-molecular test that requires minimal instrume

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Vaping involves inhaling aerosol, often called vapor, produced by an e-cigarette or similar device. Originally introduced as a smoking alternative, vaping has gained popularity, particularly among younger people. E-liquids used in vaping typically contain nicotine, flavorings, and other chemicals. Though marketed as a safer option compared to traditional smoking, concerns about health risks persist. Vaping can lead to nicotine addiction and potential lung damage, particularly with flavored or unregulated products. Some studies link vaping to respiratory an