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Diffusion-weighted imaging (DWI) can quantitatively reflect the diffusion characteristics of tissues, providing a theoretical basis for qualitative diagnosis and quantitative analysis of a disease. To characterize testicular lesions that present as a hypointense signal on magnetic resonance imaging (MRI) T2-weighted images using DWI. Study participants were divided into three groups. Group A were healthy controls (n = 35), group B included patients with mumps orchitis (n = 2, and group C included patients with seminoma (n = 15). DW