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These results indicated that the effect of spermidine and spermine on anti-aging is related with improving autophagy and mitochondrial function.BACKGROUND Forkhead box K1 (FOXK1) is a transcription factor belonging to the Forkhead box (FOX) family and is closely related to the development of various cancers, but the functional mechanism through which FOXK1 regulates autophagy and epithelial-mesenchymal transition (EMT) in the acidic microenvironment of gastric cancer (GC) remains unclear. RESULTS Our results indicated that the inhibition