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This study aimed to evaluate the prevalence of S. pneumoniae colonization in three different sites in healthy adults nasopharynx, oropharynx and gingival sulcus. Two-hundred and sixty five adults, aged 20-60 years, who attended dental clinics in one public university (n=106) and one military institution (n=159) were enrolled in this study. Pneumococcal detection was performed by direct culture (DC) and PCR for lytA gene after a broth enrichment step. Capsular types were determined by sequential multiplex PCR. We identified 18 (6.8%) pneu