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Carboxylesterase-mediated metabolism is one of major mechanisms involved in insecticide resistance. Our previous study has identified multiple carboxylesterase genes with their expression levels were significantly upregulated in pyrethroid resistant house flies. To further explore their metabolic functions, we used insect Spodoptera frugiperda (Sf9) cells to express these carboxylesterases in vitro and measure their hydrolytic activities toward esterase substrates. Our results indicated that these carboxylesterases can efficiently hydro

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