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Here, we have outlined multiple methods for inducing auxin-mediated depletion of target proteins in C. elegans, highlighting the versatility and power of this method. © 2021 Wiley Periodicals LLC. Basic Protocol 1 Long-term auxin-mediated depletion on plates Support Protocol Preparation of NGM and NGM-auxin plates Basic Protocol 2 Rapid auxin-mediated depletion via soaking Basic Protocol 3 Acute auxin-mediated depletion in isolated embryos Basic Protocol 4 Assessing auxin-mediated depletion.Myocardial infarction (MI) commonly leads to c

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