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Plasma orexin levels were determined by ELISA, following which the results of the two groups were compared, and the relevant factors were analyzed. Our results revealed significantly lower plasma orexin levels in the narcolepsy patients compared to in healthy subjects. There were no correlations between plasma orexin levels and sex, age, body mass index, disease duration, and the severity of sleepiness, and between plasma orexin levels and electrophysiological indicators, including mean sleep latency and sleep-onset rapid e