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Conventional dendritic cells (cDC) are bone marrow derived immune cells that play a central role in linking innate and adaptive immunity. cDCs efficiently uptake, process, and present antigen to naïve T cells, driving clonal expansion of antigen specific T cell responses. In chicken, vital reagents are lacking for the efficient and precise identification of cDCs. In this study we have developed several novel reagents for the identification and characterisation of chicken cDCs. Chicken FLT3 cDNA was cloned and a monoclonal antibody to ce