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The majority of analysed studies used HNO3 for the sample decomposition, with the majority of analyses being performed using atomic absorption spectroscopy (53%). Mainly blank controls (19%), analyte recovery (18%) and replicates (18%) were used as methods of validating analytical procedures. Furthermore, the studies used certified reference materials, which measure the accuracy of the methods used. We conclude that the increase in the use of aquatic reptiles in environmental monitoring research is mainly due to their abili