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The facile fabrication of covalent organic frameworks (COFs) is significant for exploring and promoting their application. In this study, TAPA-TFPB-COFs with flower-shaped morphology, good crystallinity, and high surface area was prepared via a facile room-temperature method. The as-synthesized TAPA-TFPB-COFs exhibited great adsorption capacity towards quinolone antibiotics (QAs) and satisfying reusability. Utilizing the TAPA-TFPB-COFs as solid-phase extraction cartridge packing and liquid chromatography-tandem mass s