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Data were analysed by ANOVA and Tukey tests (α=0.05). The comparison of DTS in pH groups were 8.47.49.46.45.44.4 (P0.05); and in bismuth oxide groups were fine particles medium particles coarse particles (P0.05). Acidic pH, negatively affected the distribution of Ca2+ and Si4+ ions, while bismuth oxide with fine particles enhanced it. Acidic pH can decline the DTS of MTA significantly. However, reducing the particle size of bismuth oxide can increase the DTS of MTA significantly. Acidic pH can