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No intraspecific sequence variation of partial 28S rDNA was observed between specimens from the two sampling sites. Presence of another North-American ancyrocephalid parasite Onchocleidus dispar was confirmed in pumpkinseed sunfish at both sites, and in largemouth bass from the Coruche Reservoir, this representing the first record of O. dispar on largemouth bass in Europe. Conclusion Onchocleidus principalis has been confirmed to parasitise both centrarchid fish species introduced to Europe. Compared to other studies, the species collec

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