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Mycoplasma bovis, a cattle pathogen of major economic importance across the globe, causes a range of diseases, including pneumonia and mastitis. Because of the limited options for effective treatment of these diseases, prevention and control are preferred to diagnosis and treatment. In this study, the efficacies of citric acid and sodium hypochlorite as disinfectants against M. bovis were tested using a modification of a standardised method for assessing the efficacy of disinfectants against bacteria. A citric acid concentration of 0.5