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The ultrastructural characteristics of the mature spermatozoon of Holorchis pycnoporus (Digenea, Lepocreadioidea, Aephnidiogenidae) are described by means of transmission electron microscopy (TEM). Live worms were collected from the digestive tract of the Striped seabream Lithognathus mormyrus (Teleostei, Sparidae), off the Gulf of Gabès at La Chebba (Tunisia). The ultrastructural study reveals that the male gamete of H. pycnoporus is a filiform cell tapered at both extremities and exhibiting the type III of the digenean spermatozoon pro