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The mutation c.1550_1551del has not produced new splicing site, but there is a selective splicing in varying degree significantly after mutation. On the contrary, function validation assay showed that cells transfected with the mutant TSC1 plasmids expressed significantly lower TSC1 in mRNAs and proteins levels, compared with the wild-type TSC1 plasmid transfection. A translation inhibitor cycloheximide and small interfering RNA of UPF1 (siRNA-UPF1) increased mRNA or protein expression of TSC1 significantly in cells transfected with th

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2 in 1 Pushchair With Car Seat The seat can be folded using just one hand, whether it is facing the parent or the world. This is in contrast to buggies that require the seat to removed and then put back in place. The handle also has several height settings, making it suitable for parents of different heights. The pushchair has a lie-flat seat suitable for newborns and is compatible with the Cot S carrycot that is ideal for outdoor naps. It comes with a variety of accessories. Everyday 2-in-1