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AX- and Clav-specific clones required antigen presenting cells to proliferate and drugs were presented to CD4+ and CD8+ T-cell by MHC class-II and I, respectively. A higher secretion of IL-13 and IL-5 was detected in presence of the culprit drug compared with the alternative drug. Clones expressed CD69, CCR4, CXCR3 and CCR10. CONCLUSIONS Our study details the antigen specificity and phenotype of T-cell clones generated from patients with AX-Clav-induced immediate hypersensitivity diagnosed by positive skin test. AX- and Clav-specific clon

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t we validated in the current study can be readily used by clinicians, such as pediatricians, as part of screening procedures to quickly identify whether children might be at risk for secondhand smoking exposure. The current study used a rigorous data-driven approach to identify questions that could reliably predict secondhand smoking exposure (SHS) among children.Using saliva cotinine concentration levels as a gold standard for determining SHS exposure, our analysis employing elastic net regression identified two questions tha