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The competitive binding assays confirmed that MperOBP3, MperOBP7, MperOBP9 and MperOBP3/7/9 mixture exhibited a stronger binding affinity for EβF, than for sex pheromones and plant volatiles with a dissociation constant of 2.5 μM, 1.1 μM, 3.9 μM and 1.0 μM, respectively. The competitive binding curve of MperOBP3/7/9 mixture to EβF is shallow without bottom plateau, suggesting a conformational change and a rapid dissociation after the displacement of all 1-NPN (in vivo after the saturation binding of all OBPs by EβF). The interaction betwe

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