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The data can then be freely navigated while the software automatically selects, loads, and displays the most appropriate detail. lcmsWorld is open source. Releases, source code, and example data files are available via https//github.com/PGB-LIV/lcmsWorld.Here we report a scalable synthesis of the key amino acid residue, (2S,3R,4R)-4,5-dihydroxyisoleucine (DHIle) in α-amanitin, that in turn enables the scalable synthesis of an equipotent analogue, Asn(N-ethylazide)-S,6'-dideoxy-α-amanitin, suitable for CuAAC conjugation to empower stu