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Regulated expression of genetic elements that either encode polypeptides or various types of functional RNA is a fundamental goal for gene therapy. Inducible expression may be preferred over constitutive promoters to allow clinician-based control of gene expression. Existing Tet-On systems represent one of the tightest rheostats for control of gene expression in mammals. However, basal expression in absence of tetracycline compromises the widespread application of Tet-controlled systems in gene therapy. We demonstrate that the order o

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Vehicle Key Repairs You Shouldn't Do Yourself It can be a major pain when your car keys stop working. This can be due to many reasons. The positive side is that the majority of vehicle keys can be fixed without visiting a dealership or paying for locksmith. Here are some typical issues and the easy fixes. Keys lost or damaged Keys You want your car to be running quickly after a key break. However, attempting DIY repairs could exacerbate the situation. For instance, if a key is broken inside the ignition