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Exo III would cut these double-stranded DNA to release Rox and Hg2+, thereby impeding the ET effect and recovering the fluorescent of Rox. Such SiQDs/Rox-DNA/Exo III ratiometric fluorescent sensing platform exhibited a linear response concentration range of 0.02 nM-10 nM with a detection limit of 0.01 nM. It was successfully used to analyze the water and soil samples. The reliability was validated by ICP-MS. Our work should promote the practical application of ratiometric fluorescent assay.Nucleic acid-based molecular diagnosis has

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Notably, among various immune cells and their clusters with distinguishable metabolic features, those with immunosuppressive function presented higher metabolic activities. Collectively, we uncovered the heterogeneity in energy metabolism using a classifier with prognostic and therapeutic value. Single-cell transcriptome profiling provided novel metabolic insights that could ultimately tailor therapeutic strategies based on patient- or cell type-specific cancer metabolism.This study aimed at investigating whether 1) different sinusoida