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To evaluate in a laboratory study, the effect of different canal irrigant solutions and activation techniques on transforming growth factor (TGF-β1), insulin growth factor-1 (IGF-1), bone morphogenetic protein-7 (BMP-7) and vascular endothelial growth factor-A (VEGF-A) release levels from the dentine of extracted premolar teeth. Seventy premolar teeth with single root and canal were used. The lengths of the root segments were standardized to 12mm, and the root canals were prepared up to size 100 with hand files. All surfaces of the

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