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Amphibians are the most important vulnerable non-target vertebrate group that are affected by pesticides. Most previous studies have confirmed the destructive effects of pesticides. But, so far, no comprehensive studies have been carried out in Iran. Therefore, to estimate the mortality rate of frogs during the growing season in different cultivating systems, we examined the presence of pesticides in water and substrate as indicators of habitat quality and in the liver tissue of Marsh frog Pelophylax ridibundus (Pallas, 1