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Unlike animals, which have just one KISS gene, two paralogous genes, kiss1 and kiss2, have already been identified in zebrafish along with other non-mammalian vertebrates. Past researches claim that Kiss2, not Kiss1, is the reproduction relevant form amongst the two. To better understand the role of every of these isoforms in reproduction, a loss of function strategy had been used. Two genetic manipulation techniques-clustered frequently interspaced quick palindromic repeats (CRISPR) and transcriptio

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