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In addition, the environmentally benign methodology for the synthesis of 3at effectively shortens the time from laboratory-scale research to practical applications.The benefits and disadvantages of hydrochar incorporation into soil have been heavily researched. However, the effect of hydrochar application on the soil microbial communities and the molecular structure of native soil organic carbon (SOC) has not been thoroughly elucidated. This study conducted an incubation experiment at 25 °C for 135 days using a soil column with 0.5 and

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Reverse transcription quantitative PCR (RT-qPCR) to quantify gene expression is a key molecular technique for the detection of mRNAs due to amplification of transcripts that may be present in low abundance. The technique also permits detection of mRNAs for different isoforms of proteins due to the exquisite specificity of carefully designed primers. Here I describe the detection of mRNAs for VEGF isoforms in mouse, rat and zebrafish. The protocol may be adapted for detection of any mRNAs by designing primers specific to the genes of