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Results The highest cell viability was observed in the NPEP group (p0.05). Conclusion Removing the residual EDTA using PBS improved the cell viability on the dentin surface. Ultrasonic activation enhanced the growth factor release and biological properties while the preparation of EDTA with NBs showed a similar effect to regular EDTA without compromising cellular effect.Medium-chain length methyl ketones are potential blending fuels due to their cetane numbers and low melting temperatures. Biomanufacturing offers the potential to produ