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Buy a German Shepherd Puppy From a Reputable Breeder The purchase of a German Shepherd puppy is a serious commitment. Before you purchase a puppy it is important to conduct some research on breeders who are reputable. Verify if of the litter have been certified as being free of degenerative myelopathy, megaesophagus. Exocrine insufficiency, megaes Von Willebrand's Disease. Ask about the breeder's return policy. Breeders with good reputations are willing to return their dogs if they don't fit into your lifestyle. Find a Reputable breeder The last thing you want to d

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Powdery mildew (PM) is a severe fungal disease of cucumber worldwide. Identification of genetic factors resistant to PM is of great importance for marker-assisted breeding to ensure cucumber production. Long noncoding RNAs (lncRNAs) and miRNAs have been shown to play important roles in plant development and immunity; however, whether they have a role in PM response in cucurbit crops remains unknown. Here, we performed strand-specific RNA sequencing and miRNA sequencing using RNA from cucumber leaves of two near-isogenic lines (NILs) S1