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0% 100% respectively (p less then 0.001) with 100% genotype-specific concordance. The kappa agreement with cervical samples was fair (0.25, p = 0.16). For urine hrHPV-mRNA detection of ASC-H/HSIL when grouped by age ≥ 30, the sensitivity and specificity were 45.4% 63.9% respectively (p = 0.009). For urine hrHPV-mRNA detection of ≥ CIN 2 for all ages, the sensitivity and specificity were 45.5% 75.0% respectively (p = 0.03). Conclusion Using the Aptima Assay, urine hrHPV-mRNA detection is suboptimal for cervical cancer screening but given th